NEET MDS Lessons
Periodontology
Acquired Pellicle in the Oral Cavity
The acquired pellicle is a crucial component of oral health, serving as the first line of defense in the oral cavity and playing a significant role in the initial stages of biofilm formation on tooth surfaces. Understanding the composition, formation, and function of the acquired pellicle is essential for dental professionals in managing oral health.
Composition of the Acquired Pellicle
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Definition:
- The acquired pellicle is a thin, organic layer that coats all surfaces in the oral cavity, including both hard (tooth enamel) and soft tissues (gingiva, mucosa).
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Components:
- The pellicle consists of more than 180 peptides, proteins,
and glycoproteins, which include:
- Keratins: Structural proteins that provide strength.
- Mucins: Glycoproteins that contribute to the viscosity and protective properties of saliva.
- Proline-rich proteins: Involved in the binding of calcium and phosphate.
- Phosphoproteins: Such as statherin, which helps in maintaining calcium levels and preventing mineral loss.
- Histidine-rich proteins: May play a role in buffering and mineralization.
- These components function as adhesion sites (receptors) for bacteria, facilitating the initial colonization of tooth surfaces.
- The pellicle consists of more than 180 peptides, proteins,
and glycoproteins, which include:
Formation and Maturation of the Acquired Pellicle
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Rapid Formation:
- The salivary pellicle can be detected on clean enamel surfaces within 1 minute after exposure to saliva. This rapid formation is crucial for protecting the enamel and providing a substrate for bacterial adhesion.
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Equilibrium State:
- By 2 hours, the pellicle reaches a state of equilibrium between adsorption (the process of molecules adhering to the surface) and detachment. This dynamic balance allows for the continuous exchange of molecules within the pellicle.
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Maturation:
- Although the initial pellicle formation occurs quickly, further maturation can be observed over several hours. This maturation process involves the incorporation of additional salivary components and the establishment of a more complex structure.
Interaction with Bacteria
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Bacterial Adhesion:
- Bacteria that adhere to tooth surfaces do not contact the enamel directly; instead, they interact with the acquired enamel pellicle. This interaction is critical for the formation of dental biofilms (plaque).
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Active Role of the Pellicle:
- The acquired pellicle is not merely a passive adhesion matrix. Many
proteins within the pellicle retain enzymatic activity when
incorporated. Some of these enzymes include:
- Peroxidases: Enzymes that can break down hydrogen peroxide and may have antimicrobial properties.
- Lysozyme: An enzyme that can lyse bacterial cell walls, contributing to the antibacterial defense.
- α-Amylase: An enzyme that breaks down starches and may influence the metabolism of adhering bacteria.
- The acquired pellicle is not merely a passive adhesion matrix. Many
proteins within the pellicle retain enzymatic activity when
incorporated. Some of these enzymes include:
Clinical Significance
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Role in Oral Health:
- The acquired pellicle plays a protective role by providing a barrier against acids and bacteria, helping to maintain the integrity of tooth enamel and soft tissues.
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Biofilm Formation:
- Understanding the role of the pellicle in bacterial adhesion is essential for managing plaque-related diseases, such as dental caries and periodontal disease.
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Preventive Strategies:
- Dental professionals can use knowledge of the acquired pellicle to develop preventive strategies, such as promoting saliva flow and maintaining good oral hygiene practices to minimize plaque accumulation.
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Therapeutic Applications:
- The enzymatic activities of pellicle proteins can be targeted in the development of therapeutic agents aimed at enhancing oral health and preventing bacterial colonization.
Bone grafting is a critical procedure in periodontal and dental surgery, aimed at restoring lost bone and supporting the regeneration of periodontal tissues. Various materials can be used for bone grafting, each with unique properties and applications.
A. Osseous Coagulum
- Composition: Osseous coagulum is a mixture of bone dust and blood. It is created using small particles ground from cortical bone.
- Sources: Bone dust can be obtained from various
anatomical sites, including:
- Lingual ridge of the mandible
- Exostoses
- Edentulous ridges
- Bone distal to terminal teeth
- Application: This material is used in periodontal surgery to promote healing and regeneration of bone in areas affected by periodontal disease.
B. Bioactive Glass
- Composition: Bioactive glass consists of sodium and calcium salts, phosphates, and silicon dioxide.
- Function: It promotes bone regeneration by forming a bond with surrounding bone and stimulating cellular activity.
C. HTR Polymer
- Composition: HTR Polymer is a non-resorbable, microporous, biocompatible composite made from polymethyl methacrylate (PMMA) and polyhydroxymethacrylate.
- Application: This material is used in various dental and periodontal applications due to its biocompatibility and structural properties.
D. Other Bone Graft Materials
- Sclera: Used as a graft material due to its collagen content and biocompatibility.
- Cartilage: Can be used in certain grafting procedures, particularly in reconstructive surgery.
- Plaster of Paris: Occasionally used in bone grafting, though less common due to its non-biological nature.
- Calcium Phosphate Biomaterials: These materials are osteoconductive and promote bone healing.
- Coral-Derived Materials: Natural coral can be processed to create a scaffold for bone regeneration.
Hypercementosis
Hypercementosis is a dental condition characterized by the excessive deposition of cementum on the roots of teeth. This condition can have various clinical implications and is associated with several underlying factors. Understanding hypercementosis is essential for dental professionals in diagnosing and managing related conditions.
Characteristics of Hypercementosis
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Definition:
- Hypercementosis is defined as a generalized thickening of the cementum, often accompanied by nodular enlargement of the apical third of the root. It can also manifest as spike-like excrescences known as cemental spikes.
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Forms of Hypercementosis:
- Generalized Type: Involves a uniform thickening of cementum across multiple teeth.
- Localized Type: Characterized by nodular
enlargements or cemental spikes, which may result from:
- Coalescence of cementicles adhering to the root.
- Calcification of periodontal fibers at their insertion points into the cementum.
Radiographic Appearance
- Radiographic Features:
- On radiographs, hypercementosis is identified by the presence of a radiolucent shadow of the periodontal ligament and a radiopaque lamina dura surrounding the area of hypercementosis, similar to normal cementum.
- Differentiation:
- Hypercementosis can be differentiated from other conditions such as periapical cemental dysplasia, condensing osteitis, and focal periapical osteopetrosis, as these entities are located outside the shadow of the periodontal ligament and lamina dura.
Etiology of Hypercementosis
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Varied Etiology:
- The exact cause of hypercementosis is not completely understood, but
several factors have been identified:
- Spike-like Hypercementosis: Often results from excessive tension due to orthodontic appliances or occlusal forces.
- Generalized Hypercementosis: Can occur in
various circumstances, including:
- Teeth Without Antagonists: In cases where teeth lack opposing teeth, hypercementosis may develop as a compensatory mechanism to keep pace with excessive tooth eruption.
- Low-Grade Periapical Irritation: Associated with pulp disease, where hypercementosis serves as compensation for the loss of fibrous attachment to the tooth.
- The exact cause of hypercementosis is not completely understood, but
several factors have been identified:
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Systemic Associations:
- Hypercementosis may also be observed in systemic conditions,
including:
- Paget’s Disease: Characterized by hypercementosis of the entire dentition.
- Other Conditions: Acromegaly, arthritis, calcinosis, rheumatic fever, and thyroid goiter have also been linked to hypercementosis.
- Hypercementosis may also be observed in systemic conditions,
including:
Clinical Implications
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Diagnosis:
- Recognizing hypercementosis is important for accurate diagnosis and treatment planning. Radiographic evaluation is essential for distinguishing hypercementosis from other dental pathologies.
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Management:
- While hypercementosis itself may not require treatment, it can complicate dental procedures such as extractions or endodontic treatments. Understanding the condition can help clinicians anticipate potential challenges.
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Monitoring:
- Regular monitoring of patients with known systemic conditions associated with hypercementosis is important to manage any potential complications.
Periodontal Bone Grafts
Bone grafting is a critical procedure in periodontal surgery, aimed at restoring lost bone and supporting the regeneration of periodontal tissues.
1. Bone Blend
Bone blend is a mixture of cortical or cancellous bone that is procured using a trephine or rongeurs, placed in an amalgam capsule, and triturated to achieve a slushy osseous mass. This technique allows for the creation of smaller particle sizes, which enhances resorption and replacement with host bone.
Particle Size: The ideal particle size for bone blend is approximately 210 x 105 micrometers.
Rationale: Smaller particle sizes improve the chances of resorption and integration with the host bone, making the graft more effective.
2. Types of Periodontal Bone Grafts
A. Autogenous Grafts
Autogenous grafts are harvested from the patient’s own body, providing the best compatibility and healing potential.
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Cortical Bone Chips
- History: First used by Nabers and O'Leary in 1965.
- Characteristics: Composed of shavings of cortical bone removed during osteoplasty and ostectomy from intraoral sites.
- Challenges: Larger particle sizes can complicate placement and handling, and there is a potential for sequestration. This method has largely been replaced by autogenous osseous coagulum and bone blend.
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Osseous Coagulum and Bone Blend
- Technique: Intraoral bone is obtained using high- or low-speed round burs and mixed with blood to form an osseous coagulum (Robinson, 1969).
- Advantages: Overcomes disadvantages of cortical bone chips, such as inability to aspirate during collection and variability in quality and quantity of collected bone.
- Applications: Used in various periodontal procedures to enhance healing and regeneration.
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Intraoral Cancellous Bone and Marrow
- Sources: Healing bony wounds, extraction sockets, edentulous ridges, mandibular retromolar areas, and maxillary tuberosity.
- Applications: Provides a rich source of osteogenic cells and growth factors for bone regeneration.
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Extraoral Cancellous Bone and Marrow
- Sources: Obtained from the anterior or posterior iliac crest.
- Advantages: Generally offers the greatest potential for new bone growth due to the abundance of cancellous bone and marrow.
B. Bone Allografts
Bone allografts are harvested from donors and can be classified into three main types:
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Undermineralized Freeze-Dried Bone Allograft (FDBA)
- Introduction: Introduced in 1976 by Mellonig et al.
- Process: Freeze drying removes approximately 95% of the water from bone, preserving morphology, solubility, and chemical integrity while reducing antigenicity.
- Efficacy: FDBA combined with autogenous bone is more effective than FDBA alone, particularly in treating furcation involvements.
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Demineralized (Decalcified) FDBA
- Mechanism: Demineralization enhances osteogenic potential by exposing bone morphogenetic proteins (BMPs) in the bone matrix.
- Osteoinduction vs. Osteoconduction: Demineralized grafts induce new bone formation (osteoinduction), while undermineralized allografts facilitate bone growth by providing a scaffold (osteoconduction).
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Frozen Iliac Cancellous Bone and Marrow
- Usage: Used sparingly due to variability in outcomes and potential complications.
Comparison of Allografts and Alloplasts
- Clinical Outcomes: Both FDBA and DFDBA have been compared to porous particulate hydroxyapatite, showing little difference in post-treatment clinical parameters.
- Histological Healing: Grafts of DFDBA typically heal with regeneration of the periodontium, while synthetic bone grafts (alloplasts) heal by repair, which may not restore the original periodontal architecture.
Finger Rests in Dental Instrumentation
Use of finger rests is essential for providing stability and control during procedures. A proper finger rest allows for more precise movements and reduces the risk of hand fatigue.
Importance of Finger Rests
- Stabilization: Finger rests serve to stabilize the hand and the instrument, providing a firm fulcrum that enhances control during procedures.
- Precision: A stable finger rest allows for more accurate instrumentation, which is crucial for effective treatment and patient safety.
- Reduced Fatigue: By providing support, finger rests help reduce hand and wrist fatigue, allowing the clinician to work more comfortably for extended periods.
Types of Finger Rests
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Conventional Finger Rest:
- Description: The finger rest is established on the tooth surfaces immediately adjacent to the working area.
- Application: This is the most common type of finger rest, providing direct support for the hand while working on a specific tooth. It allows for precise movements and control during instrumentation.
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Cross Arch Finger Rest:
- Description: The finger rest is established on the tooth surfaces on the other side of the same arch.
- Application: This technique is useful when working on teeth that are not directly adjacent to the finger rest. It provides stability while allowing access to the working area from a different angle.
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Opposite Arch Finger Rest:
- Description: The finger rest is established on the tooth surfaces of the opposite arch (e.g., using a mandibular arch finger rest for instrumentation on the maxillary arch).
- Application: This type of finger rest is particularly beneficial when accessing the maxillary teeth from the mandibular arch, providing a stable fulcrum while maintaining visibility and access.
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Finger on Finger Rest:
- Description: The finger rest is established on the index finger or thumb of the non-operating hand.
- Application: This technique is often used in areas where traditional finger rests are difficult to establish, such as in the posterior regions of the mouth. It allows for flexibility and adaptability in positioning.
Some important points about the periodontal pocket :
·Soft tissue of pocket wall shows both proliferative & degenerative changes
·Most severe degenerative changes are seen on the lateral wall of pocket
·Plasma cells are the predominant infiltrate (80%). Others include lymphocytes &
a scattering of PMNs
·Height of junctional epithelium shortened to only 50-100µm
·Severity of degenerative changes is not linked to pocket depth
·Junctional epithelium starts to lose attachment to tooth when PMN infiltration
in junctional epithelium increases above 60%.
Pathogens Implicated in Periodontal Diseases
Periodontal diseases are associated with a variety of pathogenic microorganisms. Below is a list of key pathogens implicated in different forms of periodontal disease, along with their associations:
General Pathogens Associated with Periodontal Diseases
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Actinobacillus actinomycetemcomitans:
- Strongly associated with destructive periodontal disease.
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Porphyromonas gingivalis:
- A member of the "black pigmented Bacteroides group" and a significant contributor to periodontal disease.
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Bacteroides forsythus:
- Associated with chronic periodontitis.
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Spirochetes (Treponema denticola):
- Implicated in various periodontal conditions.
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Prevotella intermedia/nigrescens:
- Also belongs to the "black pigmented Bacteroides group" and is associated with several forms of periodontal disease.
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Fusobacterium nucleatum:
- Plays a role in the progression of periodontal disease.
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Campylobacter rectus:
- These organisms include members of the new genus Wolinella and are associated with periodontal disease.
Principal Bacteria Associated with Specific Periodontal Diseases
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Adult Periodontitis:
- Porphyromonas gingivalis
- Prevotella intermedia
- Bacteroides forsythus
- Campylobacter rectus
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Refractory Periodontitis:
- Bacteroides forsythus
- Porphyromonas gingivalis
- Campylobacter rectus
- Prevotella intermedia
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Localized Juvenile Periodontitis (LJP):
- Actinobacillus actinomycetemcomitans
- Capnocytophaga
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Periodontitis in Juvenile Diabetes:
- Capnocytophaga
- Actinobacillus actinomycetemcomitans
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Pregnancy Gingivitis:
- Prevotella intermedia
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Acute Necrotizing Ulcerative Gingivitis (ANUG):
- Prevotella intermedia
- Intermediate-sized spirochetes