MDS PREP
The biologic standard used to test the efficiency of sterilization involves the use of
1. spores of Clostridium tetani
2. streptococcus pneumoniae
3 spores of a harmless bacillus
4. infectious hepatitis virus
Microbiology
Answer: 3
The biologic standard used to test the efficiency of sterilization typically
involves the use of spores of a harmless bacillus. This method is widely
recognized in the scientific community and is based on the principle that if a
sterilization process can effectively destroy the most heat-resistant
microorganisms, it is assumed to be capable of killing all other less resistant
microbes.
The choice of using spores of a harmless bacillus is rooted in the fact that
bacterial spores are the most heat-resistant forms of microbial life. Spores are
metabolically inactive, which makes them very hardy and capable of surviving in
adverse environmental conditions for extended periods. For sterilization
validation, scientists often employ a biological indicator that contains spores
of a known heat-resistant bacterium, such as Geobacillus stearothermophilus
(formerly Bacillus stearothermophilus) or Bacillus subtilis. These organisms are
chosen because they have well-characterized resistance profiles, and their
destruction indicates that the sterilization process has achieved the necessary
lethality to eradicate all microbial life forms, including vegetative bacteria,
fungi, and viruses.
Let's examine the other options provided:
1. Spores of Clostridium tetani: While C. tetani is a spore-forming bacterium,
its spores are not commonly used as a biological indicator for sterilization
efficiency. C. tetani is a pathogen that causes tetanus, a serious disease.
However, it is not typically used for this purpose because there are safer and
more universally accepted biological indicators available.
2. Streptococcus pneumoniae: S. pneumoniae is a bacterium that can cause
pneumonia and other infections, but it is not a spore-former. Moreover, it is
generally less resistant to heat and sterilization methods compared to
spore-forming bacteria. Thus, it is not suitable as a standard for testing
sterilization efficiency.
3. Spores of a harmless bacillus: As previously mentioned, this is the most
appropriate choice for a biologic standard in sterilization testing. These
spores serve as reliable and safe indicators of sterilization efficacy because
they mimic the resistance of pathogenic spores without posing the actual risk of
infection.
4. Infectious hepatitis virus: While viruses can be highly resistant to some
sterilization methods, they are generally more sensitive to heat than bacterial
spores. Moreover, using infectious viruses as biological indicators poses
significant biosafety risks and is not a standard practice in routine
sterilization testing. For viral resistance testing, specific viruses or
virus-like particles may be used, but these are not typically employed as the
primary biological indicators for sterilization validation due to the complexity
and high containment requirements of such testing.
What is the primary cause of cutaneous anthrax?
1) Inhalation of spores
2) Entry through the skin
3) Ingestion of contaminated food
4) Contact with infected animals
Cutaneous anthrax is primarily caused by entry through the skin, often through abrasions or cuts.
Which of the following is a killed viral vaccine?
1) Sabin vaccine for poliomyelitis
2) Salk vaccine for poliomyelitis
3) MMR vaccine for measles, mumps, rubella
4) Varicella-zoster
General Microbiology Answer: 2
The Salk vaccine for poliomyelitis is a killed viral vaccine, effectively preventing the disease by using inactivated poliovirus.
Which of the following acids is intermediate in the biosynthesis the cell wall?
1. Alanyl diaminopimelic
2. L-glutamic
3 Uridylic
4. N-acetyl muramic
Microbiology
Answer: 4
N-acetyl muramic is intermediate in the biosynthesis the cell wall
Which of the following is NOT a method used to produce a recombinant hepatitis B vaccine?
1) Cloning the HBsAg gene in E. coli
2) Cloning the HBsAg gene in yeasts Saccharomyces cerevisae
3) Extracting and purifying HBsAg particles from infected human plasma
4) Using baculovirus vectors to produce HBsAg in insect cells
General Microbiology Answer: 1
While recombinant hepatitis B vaccines are produced using various methods, cloning the HBsAg gene in E. coli is not one of them. Instead, the gene is typically cloned in yeast or insect cells to produce the surface antigen for the vaccine.
In bacillary dysentery, what is the typical stool consistency?
1) Not adherent to the container
2) Adherent to the container
3) Watery
4) Mucous
In bacillary dysentery, the stool is typically adherent to the container due to the presence of mucus and pus.
The minimum and maximum temperature for normal metabolic
activity microorganisms that live on or within the human body is
1 50 C
to 300 C
2 200 C to 450
C
3. 300 C to 400
C
4. 15 C to 600
C
Microbiology Answer: 2
The minimum and maximum temperature for normal metabolic activity microorganisms that live on or within the human body is 20° C to 45°C
What is the typical pH range for optimal growth of meningococci?
1) 6.8-7.0
2) 7.4-7.6
3) 8.0-8.2
4) 7.0-7.2
The optimal pH range for the growth of meningococci is 7.4-7.6.