LYMPHOID SYSTEM

Consists of cells, tissues and organs

Protects the body against damage by foreign substances

Immuno competent cells in the lymphoid system distinguish between the bodies own molecules and foreign molecules.

The response is immunity.

lymphoid tissues have a: - reticular framework (collagen III) consisting of:  reticular cells , (indistinguishable from fibroblasts) , lymphocytes, macrophages,  antigen presenting cells, plasma cells

Each organ has special features:

Capsulated – spleen, lymph nodes, thymus

Unencapsulated – tonsils,  Peyers patches. lymphoid nodules in: - alimentary canal

- Nodules in: respiratory tract,  urinary tract, reproductive tracts

2 Types of immunity:

- Cellular: Macrophages - destroy foreign cells

- Humeral – immunoglobulins and antibodies (glycoproteins) interact with foreign substances

- cellular and humeral immune system require accessory cells like: macrophages, antigen presenting cells

Thymus

Lymphocytes develop from mesenchym. The lymphocytes then invade an epithelial premordium .The epithelial cells are pushed apart by lymphocytes. Epithelial cells remain connected through desmosomes to form the epithelial reticular cells.  Septae from the capsule divide the thymus up into incomplete lobules (0,5-2 mm ). Each lobule has a cortex which is packed with lymphocytes. In the middle of the lobule is the lighter staining medulla. The cortex and medulla are continuous. Hassall's corpuscles, consisting of flat epithelial cells, lie in the medulla .The corpuscles increase in size and number through life

Thymus cells:

- Cortex and medulla have the same cells – only their proportions differ

- The predominant cell is the T lymphocytes and precursors

- There are also epithelial reticular cells with large oval nuclei. The cells are joined by desmosomes.

- A few mesenchymal reticular cells are also present.

- There are many macrophages.

Cortex:

- Only capillaries (no other vessels)

- small lymphocytes predominate

- here they do not form nodules

- epithelial cells surround groups of lymphocytes and blood vessels

- around the capillary is a space

- forms blood thymus barrier

- Layers of the blood thymus barrier:

- capillary wall endothelium

basal lamina

little CT with macrophages

- epithelial reticular cells - basal lamina

- cytoplasm of epithelial reticular cells

Medulla:

- Stains light because of many epithelial reticular cells

- 5% of thymic lymphocytes found in medulla

- mature lymphocytes - smaller than that of cortex

- leave through venules to populate organs such as the spleen and lymph nodes

- In the medulla the covering of capillaries by epithelial reticular cells is incomplete - no barrier

- Hassall's corpuscles

- 30 - 150µm .

- consists of layers of epithelial reticular cells

- the central part of the corpuscle may only be cell remnants

- unknown function

Lymph nodes

- Encapsulated

- found throughout the body

- form filters in the lymph tracts

- lymph penetrate through afferent lymph vessels on the convex surface

- exit through efferent lymph vessels of the hilum

- capsule send trabeculae into the node to divide it up into incomplete compartments

- reticular tissue provide the super structure

- under the capsule is a cortex – the cortex is absent at the hilum

- At the centre of the node and at the hilum is a medulla

- The cortex has a subcapsular sinus and peritrabecular sinuses

The sinuses:-

- Incompletely lined by reticular cells

- Have numerous macrophages

- fibres cross the sinuses

- they slow the flow of lymph down -

- so that the macrophages can get a chance to perform their function.

Primary and secondary lymphoid nodules

- Some lymphocytes in the cortex form spherical aggregations 0,2-1 mm Ø called primary nodules (or follicles)

- They contain mainly B lymphocytes but some T- lymphocytes are also present

- A germinal centre may develop in the middle of the nodule when an antigen is present. The nodule then becomes a secondary nodule, which is:

- light staining in the centre because:

- many B lymphocytes increase in size to become plasmablasts

- plasmablasts undergo mitosis to become plasmacytes

- plasmacytes migrate to the follicular periphery and then to the medullary cords where they mature

into plasma cells that secrete antibodies into the efferent lymph.

- lymphocytes that don’t differentiate into plasma cells remain small lymphocytes and are called memory

cells – which migrate to different parts of the body

- memory cells are capable of mounting a rapid humoral response on subsequent contact with the same antigen.

- In the nodules there are also follicular dendritic cells which are:

- non phagocytic

- with cytoplasmic extensions

- trap antigens on their surface

- present it to B and T lymphocytes which then respond

Paracortical Zone

- Between adjacent nodules and between the nodules and the medulla are loosely arranged lymphocytes which form the paracortical area or deep cortical area.

- The main cell type in this area is the T lymphocyte.

- They enter the lymph node with the blood and migrate into the paracortical zone.

- T lymphocytes are stimulated when presented with an antigen by the follicular dendritic cells.

- They transform into large lymphobasts which undergo mitosis to produce activated T lymphocytes.

- These activated T lymphocytes must go to the area of antigen stimulation to perform its function.

- When this happens the paracortex expand greatly.

- Later they join the efferent lymph to leave the lymph node.

- These lymphocytes disappear when the thymus is removed - especially if done at birth

The medulla

- Consists of medulla with branching cords separated by medullary sinusses.

- Througout the medulla are trabeculae.

- The cords contain numerous B lymphocytes and plasma cells.

- A few macrophages and T lymphocytes may also be present.

- Receive and circulate lymph from the cortical sinuses.

- Medullary sinuses communicate with efferent lymph vessels.

Spleen

- Largest lymphatic organ

- Many phagocytic cells

- Filters blood

- Form activated lymphocytes which go into the blood

- Form antibodies

General structures:

- Dense CT capsule with a few smooth muscle fibres encapsulate the spleen

- The capsule is thickened at the hilum.

- Trabeculae from the hilum carry blood vessels and nerves in and out of the spleen.

- The capsule divide the spleen into incomplete compartments.

- The spleen has no lymph vessels because it is a blood filter and not a lymph filter like the lymph nodes.

Splenic pulp

- The lymph nodules are called the white pulp

- The white pulp lies in dark red tissue called red pulp

- Red pulp is composed of splenic cords (Billroth cords) which lie between sinusoids

- Reticular tissue forms the superstructure for the spleen and contains:

- reticular cells

- macrophages

Blood circulation

- The splenic artery divide as it enters the hilum

- The arteries in the trabeculae are called trabecular arteries

- The trabecular arteries give of braches into the white pulp (central arteries).

- The artery may not lie in center but is still called a central artery.

- The central arteries give off branches to the white pulp which go through the white pulp to end in the marginal sinuses on the perimeter of the white pulp.

- The central artery continues into the red pulp (called the pulp artery) where it branches into straight arteries called penicilli.

- The penicilli continue as arterial capillaries some of which are sheated by macrophages.

- The blood from the arterial capillaries flow into the red pulp sinuses that lie between the red pulp cords.

- The way the blood gets from the capillaries into the sinuses is uncertain. It can either:

- Flow directly into the sinuses - closed theory

- Or flow through the spaces between the red pulp cord cells and then enter the sinusoid - open theory.

- Presently the open theory is popular.

- From the sinusoids the blood flow into the: - Red pulp veins

- which join the trabecular veins

- to form form the splenic vein

(Trabecular veins form channels without a wall lined by endothelium in the trabeculae.)

White pulp:

- Forms a lymph tissue sheath around the central artery

- The lymphocytes around the central artery is called the periarterial lymphatic sheath (PALS).

- Which contains mainly T lymphocytes

- So the PALS is chracterized by a central artery.

- True nodules may also be present as an extension of the PALS.

- They displace the central artery so that it lies eccentric.

- Nodules normally have a germinal center and consists mainly of B lymphocytes

- Between the red and white pulp there is a marginal zone consisting of:

- Many sinuses and of  loose lymphoid tissue.

- There are few lymphocytes

- many macrophages

- lots of blood antigens which

- play a major role in immunologic activity.

Red Pulp:

- In the fresh state this tissue has a red colour because of the many erythrocytes.

- Red pulp consists of splenic sinusses separated by splenic cords (cords of Billroth).

- Between reticular cells are macrophages, lymphocytes, granulocytes and plasma cells.

- Many of the macrophages are in the process of phagocytosing damaged erythrocytes.

- The splenic sinusoids are special sinusoidal vessels in the following ways:

- It has a dilated large irregular lumen

- Spaces between unusually shaped endothelial cells permit exchange between sinusoids and adjacent tissues. (The endothelial cells are very long arranged parallel to the direction of the vessel)

- The basal lamina of the sinusoid is not continuous but form rings.

Tonsils

- Tonsils are incompletely encapsulated lymphoid tissues

- There are - Palatine tonsils

- pharyngeal tonsils

- lingual tonsils

Palatine Tonsil

- Contains dense lymphoid tissue.

- Covered by stratified squamous non-keratinized epithelium

- with an underlying CT capsule

- Crypts that enter the tissue end blind.

Lingual Tonsil

- Lie on the posterior 1/3 of the tongue.

- Crypts link up with underlying glands that flush them.

- Epithelial covering is the same as that of the palatine tonsil.

The Arteries of the Face

• The superficial arteries are derived from the external carotid arteries.

The Facial Artery

• This is the chief artery of the face.
• It arises from the external carotid artery and winds its way to the inferior border of the mandible, just anterior to the masseter muscle.
• It hooks around the inferior border of the mandible and grooves the bone. Here the artery is superficial, just beneath the platysma and its pulsation can be felt.

• In its course over the face to the medial angle of the eye, the facial artery crosses the mandible, buccinator muscle, and maxilla.
• It lies deep to the zygomaticus major.

• The facial artery ends by sending branches to the lip and side of the nose.
• The part of the artery that runs along the side of the nose to supply the eyelids is called the angular artery.

The Superficial Temporal Artery

• This artery is the smaller of the two terminal branches of the external carotid artery (the other is the maxillary artery).
• It begins deep to the parotid gland, posterior to the neck of the mandible, and ascends superficial to the posterior end of the zygomatic process of the temporal bone. It then enters the temporal fossa.

• The superficial temporal artery ends in the scalp by dividing into the frontal and parietal branches.

• Pulsation of this artery can be felt by compressing the root of the zygomatic process of the temporal bone.

The Transverse Facial Artery

• This small artery arises from the superficial temporal artery before it emerges from the parotid gland.
• It crosses the face superficial to the masseter muscle, about a fingerbreadth inferior to the zygomatic arch.
• It divides into numerous branches that supply the parotid gland and duct, the masseter muscle, and the skin of the face.

• It anastomoses with branches of the facial artery.

Nerves of the Palate

• The sensory nerves of the palate, which are branches of the pterygopalatine ganglion, are the greater and lesser palatine nerves.
• They accompany the arteries through the greater and lesser palatine foramina, respectively.

• The greater palatine nerve supplies the gingivae, mucous membrane, and glands of the hard palate.

• The lesser palatine nerve supplies the soft palate.

• Another branch of the pterygopalatine ganglion, the nasopalatine nerve, emerges from the incisive foramen and supplies the mucous membrane of the anterior part of the hard palate.

Vessels of the Palate

• The palate has a rich blood supply from branches of the maxillary artery.

- The forehead is formed by the smooth, broad, convex plate of bone called the frontal squama.
- In foetal skulls, the halves of the frontal squama are divided by a metopic suture.
- In most people, the halves of the frontal bone begin to fuse during infancy and the suture between is usually not visible after 6 years of age.
- The frontal bone forms the thin roof of the orbits (eye sockets).
- Just superior to and parallel with each supraorbital margin is a bony ridge, the superciliary arch, which overlies the frontal sinus. This arch is more pronounced in males.
- Between these arches there is a gently, rounded, medial elevation called the gabella; this term derives from the Latin word glabellus meaning smooth and hairless. In most people, the skin over the gabella is hairless.
-The slight prominences of the forehead on each side, superior to the superciliary arches, are called frontal eminences (tubers).
- The supraorbital foramen (occasionally a notch), which transmits the supraorbital vessels and nerve is located in the medial part of the supraorbital margin.
- The frontal bone articulates with the two parietal bones at the coronal suture.
-It also articulates with the nasal bones at the frontonasal suture. At the point where this suture crosses the internasal suture in the medial plane, there is an anthropological landmark called the nasion . The depression is located at the root of the nose, where it joins the cranium.
- The frontal bone also articulates with the zygomatic, lacrimal, ethmoid, and sphenoid bones.
In about 8% of adult skulls, a remnant of the inferior part of the metopic (interfrontal) suture is visible. It may be mistaken in radiographs for a fracture line by inexperienced observers.
- The superciliary arches are relatively sharp ridges of bone and a blow to them may lacerate the skin and cause bleeding.
- Bruising of the skin over a superciliary arch causes tissue fluid and blood to accumulate in the surrounding connective tissue, which gravitates into the upper eyelid and around the eye. This results in swelling and a "black eye".
- Compression of the supraorbital nerve as it emerges from its foramen causes considerable pain, a fact that may be used by anaesthesiologists and anaesthetists to determine the depth of anaesthesia and by physicians attempting to arouse a moribund patient.

Innervation of the Pharynx

• The motor and most of the sensory supply of the pharynx is derived from the pharyngeal plexus of nerves on the surface of the pharynx.
• The plexus is formed by pharyngeal branches of the vagus (CN X) and glossopharyngeal (CN IX) nerves, and by sympathetic branches for the superior cervical ganglion.

• The motor fibres in the pharyngeal plexus are derived from the cranial root of accessory nerve (CN XI), and are carried by the vagus nerve to all muscles of the pharynx and soft palate.
• The exceptions are stylopharyngeus (supplied by CN IX) and the tensor veli palatini (supplied by CN V3).

The Articular Capsule

• The capsule of this joint is loose.
• The thin fibrous capsule is attached to the margins of the articular area on the temporal bone and around the neck of the mandible.