The pre-dentition period.

-This is from birth to six months.

-At this stage, there are no teeth. Clinically, the infant is edentulous

-Both jaws undergo rapid growth; the growth is in three planes of space: downward, forward, and laterally (to the side). Forward growth for the mandible is greater.

-The maxillary and mandibular alveolar processes are not well developed at birth.

-occasionally, there is a neonatal tooth present at birth. It is a supernumerary and is often lost soon after birth.

-At birth, bulges in the developing alveoli precede eruption of the deciduous teeth. At birth, the molar pads can touch.

Root Formation and Obliteration

1. In general, the root of a deciduous tooth is completely formed in just about one year after eruption of that tooth into the mouth.

2. The intact root of the deciduous tooth is short lived. The roots remain fully formed only for about three years.

3. The intact root then begins to resorb at the apex or to the side of the apex, depending on the position of the developing permanent tooth bud.

4. Anterior permanent teeth tend to form toward the lingual of the deciduous teeth, although the canines can be the exception. Premolar teeth form between the roots of the deciduous molar teeth

Dentin

1. Composition

a. Inorganic (70%)—calcium hydroxyapatite crystals.

b. Organic (30%)—water and type I collagen.

2. Types of dentin

a. Primary dentin

(1) Dentin formed during tooth development, before completion of root formation.

It constitutes the majority of dentin found in a tooth.

(2) It consists of a normal organization of dentinal tubules.

(3) Circumpulpal dentin

(a) The layer of primary dentin that surrounds the pulp chamber.  It is formed after the mantle dentin.

(b) Its collagen fibers are parallel to the DEJ.

b. Secondary dentin

(1) Dentin formed after root formation is complete.

(2) Is deposited unevenly around the pulp chamber, forming along the layer of dentin closest to the pulp.

It therefore contributes to the decrease in the size of the pulp chamber as one ages.

(3) It consists of a normal, or slightly less regular, organization of dentinal tubules. However,

as compared to primary dentin, it is deposited at a slower rate.

(4) Although the dentinal tubules in secondary dentin can be continuous with those in primary

dentin, there is usually a tubular angle change between the two layers.

c. Tertiary (reparative, reactive) dentin

(1) Dentin that is formed in localized areas in response to trauma or other stimuli such as caries, tooth wear, or dental work.

(2) Its consistency and organization vary. It has no defined dentinal tubule pattern

d. Mantle dentin
 

(1) The outermost layer of dentin
(2) Is the first layer of dentin laid down by odontoblasts adjacent to the DEJ.

(3) Is slightly less mineralized than primary dentin.

(4) Has collagen fibers that are perpendicular to the DEJ.

(5) Dentinal tubules branch abundantly in this area.

e. Sclerotic (transparent) dentin

(1) Describes dentinal tubules that have become occluded with calcified material .

(2) Occurs when the odontoblastic processes retreat, filling the dentinal tubule with calcium phosphate crystals.

(3) Occurs with aging.

f. Dead tracts

(1) When odontoblasts die, they leave behind empty dentinal tubules, or dead tracts.

(2) Occurs with aging or trauma.

(3) Empty tubules are potential paths for bacterial invasion.

3. Structural characteristics and microscopic features:
 

a. Dentinal tubules

(1) Tubules extend from the DEJ to the pulp chamber.

(2) The tubules taper peripherally (i.e., their diameters are wider as they get closer to the pulp). Since the tubules are distanced farther apart at the periphery, the density of tubules is greater closer to the pulp.

(3) Each tubule contains an odontoblastic process or Tomes’ fiber.

Odontoblastic processes are characterized by the presence of a network of microtubules, with

Occasional mitochondria and vesicles present.

Note: the odontoblast’s cell body remains in the pulp chamber.
 

(4) Coronal tubules follow an S-shaped path, which may result from the crowding of  odontoblasts as they migrate toward the pulp during dentin formation.

b. Peritubular dentin (intratubular dentin)

(1) Is deposited on the walls of the dentinal tubule, which affects (i.e., narrows)the diameter of the tubule .

(2) It differs from intertubular dentin by lacking a collagenous fibrous matrix. It is also more mineralized than intertubular dentin.
 

c. Intertubular dentin

(1) The main part of dentin, which fills the space between dentinal tubules

 (2) Is mineralized and contains a collagenous matrix.

d. Interglobular dentin

(1) Areas of hypomineralized or unmineralized dentin caused by the failure of globules or calcospherites to fuse uniformly with mature dentin.

(2) Dentinal tubules are left undisturbed as they pass through interglobular dentin; however,

No peritubular dentin is present.

(3) Interglobular dentin is found in the:

(a) Crown—just beneath the mantle dentin.

(b) Root—beneath the dentinocemental junction, giving the root the appearance of a granular

layer (of Tomes).

e. Incremental lines

(1) Dentin is deposited at a daily rate of approximately 4 microns.

(2) As dentin is laid down, small differences in collagen fiber orientation result in the formation of incremental lines.

(3) Called imbrication lines of von Ebner.

(a) Every 5 days, or about every 20 µm, the changes in collagen fiber orientation appear more

accentuated. This results in a darker staining line, known as the imbrication line of von

Ebner.

(b) These lines are similar to the lines of Retzius seen in enamel.

f. Contour lines of Owen

(1) An optical phenomenon that occurs when the secondary curvatures of adjacent dentinal tubules coincide, resulting in the appearance of lines known as contour lines of Owen.
 

(2) Contour lines of Owen may also refer to lines that appear similar to those just described; however, these lines result from disturbances in mineralization.

g. Granular layer of Tomes

(1) A granular or spotty-appearing band that can be observed on the root surface adjacent to the dentinocemental junction, just beneath the cementum.

ERUPTION OF THE PERMANENT TOOTH

- At the time at which the deciduous tooth erupts the tooth bud for the permanent tooth has already been building up enamel and dentin.

- When the permanent tooth starts to erupt, pressure on the root of the deciduous tooth causes resorption by the osteoclasts.

- Wolff's law states that when two hard tissues exert pressure on one another the softer of the tissues will be resorbed.

- The dentin and cementum of the root of the deciduous tooth is softer than the enamel of the permanent tooth that is why the root of the deciduous tooth is resorbed.

- Most permanent teeth have erupted and have been in use for 2 years before the root is completely formed.

Crown stage

Hard tissues, including enamel and dentin, develop during the next stage of tooth development. This stage is called the crown, or maturation, stage by some researchers. Important cellular changes occur at this time. In prior stages, all of the inner enamel epithelium cells were dividing to increase the overall size of the tooth bud, but rapid dividing, called mitosis, stops during the crown stage at the location where the cusps of the teeth form. The first mineralized hard tissues form at this location. At the same time, the inner enamel epithelial cells change in shape from cuboidal to columnar. The nuclei of these cells move closer to the stratum intermedium and away from the dental papilla.

The adjacent layer of cells in the dental papilla suddenly increases in size and differentiates into odontoblasts, which are the cells that form dentin. Researchers believe that the odontoblasts would not form if it were not for the changes occurring in the inner enamel epithelium. As the changes to the inner enamel epithelium and the formation of odontoblasts continue from the tips of the cusps, the odontoblasts secrete a substance, an organic matrix, into their immediate surrounding. The organic matrix contains the material needed for dentin formation. As odontoblasts deposit organic matrix, they migrate toward the center of the dental papilla. Thus, unlike enamel, dentin starts forming in the surface closest to the outside of the tooth and proceeds inward. Cytoplasmic extensions are left behind as the odontoblasts move inward. The unique, tubular microscopic appearance of dentin is a result of the formation of dentin around these extensions.

After dentin formation begins, the cells of the inner enamel epithelium secrete an organic matrix against the dentin. This matrix immediately mineralizes and becomes the tooth's enamel. Outside the dentin are ameloblasts, which are cells that continue the process of enamel formation; therefore, enamel formation moves outwards, adding new material to the outer surface of the developing tooth.

Tooth development is commonly divided into the following stages: the bud stage, the cap, the bell, and finally maturation. The staging of tooth development is an attempt to categorize changes that take place along a continuum; frequently it is difficult to decide what stage should be assigned to a particular developing tooth. This determination is further complicated by the varying appearance of different histological sections of the same developing tooth, which can appear to be different stages.

Bud stage

The bud stage is characterized by the appearance of a tooth bud without a clear arrangement of cells. The stage technically begins once epithelial cells proliferate into the ectomesenchyme of the jaw. The tooth bud itself is the group of cells at the end of the dental lamina.