NEET MDS Lessons
Dental Anatomy
Amelogenesis and Enamel
Enamel is highly mineralized: 85% hydroxyapatite crystals
Enamel formation is a two-step process
The first step produces partially mineralized enamel: 30% (secretory)
The second step: Influx of minerals, removal of water and organic matrix (maturative)
Again, dentin is the prerequisite of enamel formation (reciprocal induction)
Stratum intermedium: high alkaline phosphatase activity
Differentiation of ameloblasts: Increase in glycogen contents
Formation of the enamel matrix
Enamel proteins, enzymes, metalloproteinases, phosphatases, etc.
Enamel proteins: amelogenins (90%), enamelin, tuftelin, and amelin
Amelogenins: bulk of organic matrix
Tuftelin: secreted at the early stages of amelogenesis (area of the DE junction)
Enamelin: binds to mineral
Amelin
Mineralization of enamel
No matrix vesicles
Immediate formation of crystallites
Intermingling of enamel crystallites with dentin
"Soft" enamel is formed
Histologic changes
Differentiation of inner enamel epithelium cells. They become ameloblasts
Tomes' processes: saw-toothed appearance
Collapse of dental organ
Formation of the reduced enamel epithelium
Hard tissue formation (Amelogenesis )
Enamel formation is called amelogenesis and occurs in the crown stage of tooth development. "Reciprocal induction" governs the relationship between the formation of dentin and enamel; dentin formation must always occur before enamel formation. Generally, enamel formation occurs in two stages: the secretory and maturation stages. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage; the maturation stage completes enamel mineralization.
In the secretory stage, ameloblasts release enamel proteins that contribute to the enamel matrix, which is then partially mineralized by the enzyme alkaline phosphatase. The appearance of this mineralized tissue, which occurs usually around the third or fourth month of pregnancy, marks the first appearance of enamel in the body. Ameloblasts deposit enamel at the location of what become cusps of teeth alongside dentin. Enamel formation then continues outward, away from the center of the tooth.
In the maturation stage, the ameloblasts transport some of the substances used in enamel formation out of the enamel. Thus, the function of ameloblasts changes from enamel production, as occurs in the secretory stage, to transportation of substances. Most of the materials transported by ameloblasts in this stage are proteins used to complete mineralization. The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel has completed its mineralization.
Angle classified these relationships by using the first permanent molars
Normal or neutral occlusion (ideal):
Mesiobuccalgroove of the mandibular first molar align with the mesiobuccal cusp of the max laxy first permanent molar
ClassI malocclusion normal molar relationships with alterations to other characteristics of the occlusion such as versions, crossbites, excessive overjets, or overbites
Class II malocclusion a distal relation of the mesiobuccal groove of the mandibular first permanent molar to the mesiobuccal cusp of the maxillary first permanent molar
Division I: protruded maxillary anterior teeth
Division II: one or more maxillary anterior teeth retruded
Class III malocclusion a mesial relation of the mesiobuccal groove of the mandibular first permanent molar to the mesiobuccal cusp of the maxillary molar
Histology of the Periodontal Ligament (PDL)
Embryogenesis of the periodontal ligament
The PDL forms from the dental follicle shortly after root development begins
The periodontal ligament is characterized by connective tissue. The thinnest portion is at the middle third of the root. Its width decreases with age. It is a tissue with a high turnover rate.
FUNCTIONS OF PERIODONTIUM
Tooth support
Shock absorber
Sensory (vibrations appreciated in the middle ear/reflex jaw opening)
The following cells can be identified in the periodontal ligament:
a) Osteoblasts and osteoclasts b) Fibroblasts, c) Epithelial cells
Rests of Malassez
d) Macrophages
e) Undifferentiated cells
f) Cementoblasts and cementoclasts (only in pathologic conditions)
The following types of fibers are found in the PDL
-Collagen fibers: groups of fibers
-Oxytalan fibers: variant of elastic fibers, perpendicular to teeth, adjacent to capillaries
-Eluanin: variant of elastic fibers
Ground substance
PERIODONTAL LIGAMENT FIBERS
Principal fibers
These fibers connect the cementum to the alveolar crest. These are:
a. Alveolar crest group: below CE junction, downward, outward
b. Horizontal group: apical to ACG, right angle
c. Oblique group: numerous, coronally to bone, oblique direction
d. Apical group: around the apex, base of socket
e. Interradicular group: multirooted teeth
Gingival ligament fibers
This group is not strictly related to periodontium. These fibers are:
a. Dentogingival: numerous, cervical cementum to f/a gingiva
b. Alveologingival: bone to f/a gingiva
c. Circular: around neck of teeth, free gingiva
d. Dentoperiosteal: cementum to alv. process or vestibule (muscle)
e. Transseptal: cementum between adjacent teeth, over the alveolar crest
Blood supply of the PDL
The PDL gets its blood supply from perforating arteries (from the cribriform plate of the bundle bone). The small capillaries derive from the superior & inferior alveolar arteries. The blood supply is rich because the PDL has a very high turnover as a tissue. The posterior supply is more prominent than the anterior. The mandibular is more prominent than the maxillary.
Nerve supply
The nerve supply originates from the inferior or the superior alveolar nerves. The fibers enter from the apical region and lateral socket walls. The apical region contains more nerve endings (except Upper Incisors)
Dentogingival junction
This area contains the gingival sulcus. The normal depth of the sulcus is 0.5 to 3.0 mm (mean: 1.8 mm). Depth > 3.0 mm is considered pathologic. The sulcus contains the crevicular fluid
The dentogingival junction is surfaced by:
1) Gingival epithelium: stratified squamous keratinized epithelium 2) Sulcular epithelium: stratified squamous non-keratinized epithelium The lack of keratinization is probably due to inflammation and due to high turnover of this epithelium.
3) Junctional epithelium: flattened epithelial cells with widened intercellular spaces. In the epithelium one identifies neutrophils and monocytes.
Connective tissue
The connective tissue of the dentogingival junction contains inflammatory cells, especially polymorphonuclear neutrophils. These cells migrate to the sulcular and junctional epithelium.
The connective tissue that supports the sulcular epithelium is also structurally and functionally different than the connective tissue that supports the junctional epithelium.
Histology of the Col (=depression)
The col is found in the interdental gingiva. It is surfaced by epithelium that is identical to junctional epithelium. It is an important area because of the accumulation of bacteria, food debris and plaque that can cause periodontal disease.
Blood supply: periosteal vessels
Nerve supply: periodontal nerve fibers, infraorbital, palatine, lingual, mental, buccal
MAXILLARY CENTRAL INCISORS
Viewed mesially or distally, a maxillary central incisor looks like a wedge, with the point of the wedge at the incisal (cutting) edge of the tooth.
Facial Surface- The mesial margin is nearly straight and meets the incisal edge at almost a 90° angle, but the distal margin meets the incisal edge in a curve. The incisal edge is straight, but the cervical margin is curved like a half moon. Two developmental grooves are on the facial surface.
Lingual Surface:- The lingual aspect presents a distinctive lingual fossa that is bordered by mesial and distal marginal ridges, the incisal edge, and the prominent cingulum at the gingival. Sometimes a deep pit, the lingual pit, is found in conjunction with a cingulum.
Incisal: The crown is roughly triangular in outline; the incisal edge is nearly a straight line, though slightly crescent shaped
Contact Points: The mesial contact point is just about at the incisal, owing to the very sharp mesial incisal angle. The distal contact point is located at the junction of the incisal third and the middle third.
Root Surface:-As with all anterior teeth, the root of the maxillary central incisor is single. This root is from one and one-fourth to one and one-half times the length of the crown. Usually, the apex of the root is inclined slightly distally.
The mixed dentition
I. Transition dentition between 6 and 12 years of age with primary tooth exfoliation and permanent tooth eruption
2. Its characteristic features have led this to be called the ugly duckling stage because of
a. Edentulated areas
b. Disproportionately sized teeth
c. Various clinical crown heights
d. Crowding
e. Enlarged and edematous gingiva
f. Different tooth colors
CEMENTUM vs. BONE
Cementum simulates bone
1) Organic fibrous framework, ground substance, crystal type, development
2) Lacunae
3) Canaliculi
4) Cellular components
5) Incremental lines (also known as "resting" lines; they are produced by continuous but phasic, deposition of cementum)
Differences between cementum and bone
1) Cementum is not vascularized
2) Cementum has minor ability to remodel
3) Cementum is more resistant to resorption compared to bone
4) Cementum lacks neural component
5) Cementum contains a unique proteoglycan interfibrillar substance
6) 70% of bone is made by inorganic salts (cementum only 46%)
Relation of Cementum to Enamel at the Cementoenamel Junction (CEJ)
"OMG rule"
In 60% of the teeth cementum Overlaps enamel
In 30% of the teeth cementum just Meets enamel
In 10% of the teeth there is a small Gap between cementum and enamel
MAXILLARY FIRST MOLAR
The first molars are also known as 6-year molars, because they erupt when a child is about 6 years
Facial Surface:-The facial surface has a facial groove that continues over from the occlusal surface, and runs down to the middle third of the facial surface.
Lingual Surface:-In a great many instances, there is a cusp on the lingual surface of the mesiolingual cusp. This is a fifth cusp called the cusp of Carabelli, which is in addition to the four cusps on the occlusal surface.
Proximal: In mesial perspective the mesiolingual cusp, mesial marginal ridge, and mesiobuccal cusp comprise the occlusal outline. In its distal aspect, the two distal cusps are clearly seen; however, the distal marginal ridge is somewhat shorter than the mesial one.
Occlusal Surface:- The tooth outline is somewhat rhomboidal with four distinct cusps. The cusp order according to size is: mesiolingual, mesiobuccal, distobuccal, and distolingual. The tips of the mesiolingual, mesiobuccal, and distobuccal cusps form the trigon, Cusp of Carabelli located on the mesiolingual cusp.
Contact Points; The mesial contact is above, but close to, the mesial marginal ridge. It is somewhat buccal to the center of the crown mesiodistally. The distal contact is similarly above the distal marginal ridge but is centered buccolingually.
Roots:-The maxillary first molar has three roots, which are named according to their locations mesiofacial, distofacial, and lingual (or palatal root). The lingual root is the largest.