Enamel

Structural characteristics and microscopic features

a.  Enamel rods or prisms

(1) Basic structural unit of enamel.

(2) Consists of tightly packed hydroxyapatite crystals. Hydroxyapatite crystals in enamel are four times larger and more tightly packed than hydroxyapatite found in other calcified

tissues (i.e., it is harder than bone).

(3) Each rod extends the entire thickness of enamel and is perpendicular to the dentinoenamel junction (DEJ).
 

b. Aprismatic enamel

(1) The thin outer layer of enamel found on the surface of newly erupted teeth.

(2) Consists of enamel crystals that are aligned perpendicular to the surface.

(3) It is aprismatic (i.e., prismless) and is more mineralized than the enamel beneath it.

(4) It results from the absence of Tomes processes on the ameloblasts during the final stages of enamel deposition.

c. Lines of Retzius (enamel striae)

(1) Microscopic features

 (a) In longitudinal sections, they are observed as brown lines that extend from the DEJ to the

tooth surface.

 (b) In transverse sections, they appear as dark, concentric rings similar to growth rings in a tree.
 

(2) The lines appear weekly during the formation of enamel.
 

(3) Although the cause of striae formation is unknown, the lines may represent appositional or incremental growth of enamel. They may also result from metabolic disturbances of ameloblasts.

(4) Neonatal line

(a) An accentuated, dark line of Retzius that results from the effect of physiological changes

on ameloblasts at birth.

(b) Found in all primary teeth and some cusps of permanent first molars.

d. Perikymata

(1) Lines of Retzius terminate on the tooth surface in shallow grooves known a perikymata.

(2) These grooves are usually lost through wear but may be observed on the surfaces of developing teeth or nonmasticatory surfaces of formed teeth.
 

e. Hunter-Schreger bands

(1) Enamel rods run in different directions. In longitudinal sections, these changes in direction result in a banding pattern known as HunterSchreger bands.

(2) These bands represent an optical phenomenon of enamel and consist of a series of  alternating dark and light lines when the section is viewed with reflected or polarized

light.

f. Enamel tufts

(1) Consist of hypomineralized groups of enamel rods.

(2) They are observed as short, dark projections found near or at the DEJ.

(3) They have no known clinical significance.

g. Enamel lamellae
 

(1) Small, sheet-like cracks found on the surface of enamel that extend its entire thickness.

(2) Consist of hypocalcified enamel.

(3) The open crack may be filled with organic material from leftover enamel organ components, connective tissues of the developing tooth, or debris from the oral cavity.

(4) Both enamel tufts and lamellae may be likened to geological faults in mature enamel.
 

h. Enamel spindle
 

(1) Remnants of odontoblastic processes that become trapped after crossing the DEJ during the differentiation of ameloblasts.
 

(2) Spindles are more pronounced beneath the cusps or incisal edges of teeth (i.e., areas where occlusal stresses are the greatest).
 

Cementum

Composition

a. Inorganic (50%)—calcium hydroxyapatite crystals.

b. Organic (50%)—water, proteins, and type I collagen.

c. Note: Compared to the other dental tissues, the composition of cementum is most similar to bone; however, unlike bone, cementum is avascular (i.e., no Haversian systems or other vessels are present).

Main function of cementum is to attach PDL fibers to the root surface.

Cementum is generally thickest at the root apex and in interradicular areas of multirooted 

Types of cementum

a. Acellular (primary) cementum

(1) A thin layer of cementum that surrounds the root, adjacent to the dentin.

(2) May be covered by a layer of cellular cementum, which most often occurs in the middle and apical root.

(3) It does not contain any cells.

b. Cellular (secondary) cementum

(1) A thicker, less-mineralized layer of cementum that is most prevalent along the apical root and in interradicular (furcal) areas of multirooted teeth.

(2) Contains cementocytes.

(3) Lacunae and canaliculi:

(a) Cementocytes (cementoblasts that become trapped in the extracellular matrix during cementogenesis) are observed in their entrapped spaces, known as lacunae.

(b) The processes of cementocytes extend through narrow channels called canaliculi.

(4) Microscopically, the best way to differentiate between acellular and cellular cementum is the presence of lacunae in cellular cementum.

Alveolar bone (process)

1. The bone in the jaws that contains the teeth alveoli (sockets).

2. Three types of bone :

a. Cribriform plate (alveolar bone proper)

(1) Directly lines and forms the tooth socket. It is compact bone that contains many holes, allowing for the passage of blood vessels. It has no periosteum.

(2) Serves as the attachment site for PDL (Sharpey’s) fibers.

(3) The tooth socket is constantly being remodeled in response to occlusal forces. The bone laid down on the cribriform plate, which also provides attachment for PDL fibers, is known as bundle bone.

(4) It is radiographically known as the lamina dura.

b. Cortical (compact) bone

(1) Lines the buccal and lingual surfaces of the mandible and maxilla.

(2) Is typical compact bone with a periosteum and contains Haversian systems.

(3) Is generally thinner in the maxilla and thicker in the mandible, especially around the buccal area of  the mandibular premolar and molar.

c. Trabecular (cancellous, spongy) bone

(1) Is typical cancellous bone containing Haversian systems.

(2) Is absent in the maxillary anterior teeth region.

3. Alveolar crest (septa)

a. The height of the alveolar crest is usually 1.5 to 2 mm below the CEJ junction.

b. The width is determined by the shape of adjacent teeth.

(1) Narrow crests—found between teeth with relatively flat surfaces.

(2) Widened crests—found between teeth with convex surfaces or teeth spaced apart.

Amelogenesis and Enamel

Enamel is highly mineralized: 85% hydroxyapatite crystals
Enamel formation is a two-step process
The first step produces partially mineralized enamel: 30% (secretory)
The second step: Influx of minerals, removal of water and organic matrix (maturative)
Again, dentin is the prerequisite of enamel formation (reciprocal induction)
Stratum intermedium: high alkaline phosphatase activity
Differentiation of ameloblasts: Increase in glycogen contents

Formation of the enamel matrix
Enamel proteins, enzymes, metalloproteinases, phosphatases, etc.
Enamel proteins: amelogenins (90%), enamelin, tuftelin, and amelin
Amelogenins: bulk of organic matrix
Tuftelin: secreted at the early stages of amelogenesis (area of the DE junction)
Enamelin: binds to mineral
Amelin

Mineralization of enamel
 No matrix vesicles
Immediate formation of crystallites
Intermingling of enamel crystallites with dentin
"Soft" enamel is formed

Histologic changes

Differentiation of inner enamel epithelium cells. They become ameloblasts
Tomes' processes: saw-toothed appearance
Collapse of dental organ
Formation of the reduced enamel epithelium

Hard tissue formation (Amelogenesis )

Enamel formation is called amelogenesis and occurs in the crown stage of tooth development. "Reciprocal induction" governs the relationship between the formation of dentin and enamel; dentin formation must always occur before enamel formation. Generally, enamel formation occurs in two stages: the secretory and maturation stages. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage; the maturation stage completes enamel mineralization.

In the secretory stage, ameloblasts release enamel proteins that contribute to the enamel matrix, which is then partially mineralized by the enzyme alkaline phosphatase. The appearance of this mineralized tissue, which occurs usually around the third or fourth month of pregnancy, marks the first appearance of enamel in the body. Ameloblasts deposit enamel at the location of what become cusps of teeth alongside dentin. Enamel formation then continues outward, away from the center of the tooth.

In the maturation stage, the ameloblasts transport some of the substances used in enamel formation out of the enamel. Thus, the function of ameloblasts changes from enamel production, as occurs in the secretory stage, to transportation of substances. Most of the materials transported by ameloblasts in this stage are proteins used to complete mineralization. The important proteins involved are amelogenins, ameloblastins, enamelins, and tuftelins. By the end of this stage, the enamel has completed its mineralization.

Maxillary First Deciduous Molar.

-The notation is B or I.

-It looks a bit like an upper 1st premolar.

-There are three roots.

-It has a strong bulbous enamel bulge that protrudes buccally at the mesial.

-It is the smallest of the deciduous molars in crown height and in the mesiodistal dimension.

Mixed Dentition Period.

-Begins with the eruption of the first permanent molars distal to the second deciduous molars. These are the first teeth to emerge and they initially articulate in an 'end-on' (one on top of the other) relationship.

-On occasion, the permanent incisors spread out due to spacing. In the older literature, is called by the 'ugly duckling stage.' With the eruption of the permanent canines, the spaces often will close.

-Between ages 6 and 7 years of age there are:

20 deciduous teeth

4 first permanent molars

28 permanent tooth buds in various states of development