Periodontal ligament development

Cells from the dental follicle give rise to the periodontal ligaments (PDL).

Formation of the periodontal ligaments begins with ligament fibroblasts from the dental follicle. These fibroblasts secrete collagen, which interacts with fibers on the surfaces of adjacent bone and cementum. This interaction leads to an attachment that develops as the tooth erupts into the mouth. The occlusion, which is the arrangement of teeth and how teeth in opposite arches come in contact with one another, continually affects the formation of periodontal ligaments. This perpetual creation of periodontal ligaments leads to the formation of groups of fibers in different orientations, such as horizontal and oblique fibers.

Cementum

Composition

a. Inorganic (50%)—calcium hydroxyapatite crystals.

b. Organic (50%)—water, proteins, and type I collagen.

c. Note: Compared to the other dental tissues, the composition of cementum is most similar to bone; however, unlike bone, cementum is avascular (i.e., no Haversian systems or other vessels are present).

Main function of cementum is to attach PDL fibers to the root surface.

Cementum is generally thickest at the root apex and in interradicular areas of multirooted 

Types of cementum

a. Acellular (primary) cementum

(1) A thin layer of cementum that surrounds the root, adjacent to the dentin.

(2) May be covered by a layer of cellular cementum, which most often occurs in the middle and apical root.

(3) It does not contain any cells.

b. Cellular (secondary) cementum

(1) A thicker, less-mineralized layer of cementum that is most prevalent along the apical root and in interradicular (furcal) areas of multirooted teeth.

(2) Contains cementocytes.

(3) Lacunae and canaliculi:

(a) Cementocytes (cementoblasts that become trapped in the extracellular matrix during cementogenesis) are observed in their entrapped spaces, known as lacunae.

(b) The processes of cementocytes extend through narrow channels called canaliculi.

(4) Microscopically, the best way to differentiate between acellular and cellular cementum is the presence of lacunae in cellular cementum.

Histology of the Pulp

PARTICIPATING CELLS

1. Odontoblasts (body and process)
Most distinctive cells of the pulp
Single layer
The cells are columnar in the coronal portion, cuboidal in the middle portion, flat in the apical portion

Individual odontoblasts communicate with each other via junctions. The number of odontoblasts corresponds to the number of dentinal tubules.
The lifespan of an odontoblast equals the one of a vital tooth.
The morphology of the odontoblasts reflects their functional activity.
(There are three stages that reflect the functional activity of a cell: active, transitional and resting)

The odontoblastic process

2. Fibroblasts
Most numerous cells
Produce collagen fibers and ground substance
Ground substance consists of: proteoglycans and glycoproteins
Again, active and resting cells
Fibroblasts have also capability to degrade collagen

3. Undifferentiated mesenchymal cells A pool of cells from which connective tissue cells can derive.
They are reduced with age.

4. Endothelial cells, Schwann cells, pericytes and immunocompetent cells

MATRIX

It is composed of fibers and ground substance
55% of the fibers are Type I collagen. 45% of the fibers are Type III collagen.
The ground substance is gelatinous in the coronal aspect and more fibrous in the apical.

VASCULARITY

Superior and inferior alveolar arteries that derive from the external carotids
Afferent side of the circulation: arterioles
Efferent side of the circulation: venules
Lymphatics

Small, blind, thin-walled vessels in the coronal region of the pulp and exit via one or two larger vessels.
 

ERUPTION

. Root completion (approximately 50% of the root is formed when eruption begins)

Generally mandibular teeth erupt before maxillary teeth,

Primary teeth

I. Emerge into the oral cavity as follows:

           Maxillary                       Mandibular

Central Incisor                          7½ months                     6 months

Lateral incisor                           9 months                       7 months

Canine                                     18 months                      16 months

First Molar                               14 months                     12 months

Second Molar                          24months                       20 months

The sequence of  primary  tooth development is central incisor, lateral incisor, first molar, second molar

3. Hard tissue formation begins between 4 and 6 months in utero

4. Crowns completed between 1½ and 10 months of age

5. Roots are completed between I½ and3 yearsof age 6 to 18 months after eruption

6. By age 3 years all of the primary and permanent teeth (except for the third molars) are in some stage of development

7. Root resorption of primary teeth is triggered by the pressure exerted by the developing permanent tooth; it is followed by primary tooth exfoliation in sequential patterns

8. The primary dentition ends when the first permanent tooth erupts