The very first histological evidence of tooth development appear during the second month of intrauterine life. Calcification of deciduous incisors begins at 3-4 months in utero.

HISTOLOGY OF THE ODONTOBLAST

Formation of Dentin

Mantle dentin: First formed dentin
Type I collagen with ground substance
Formation of the odontoblast process

Matrix vesicles
Appearance of hydroxyapatite crystals
 

Predentin
Primary physiologic (circumpulpal) dentin
All organic matrix is formed from odontoblasts
Smaller collagen fibers
Presence of phosphophoryn

Mineralization
Globular calcification
Interglobular dentin: Areas of incomplete calcification
Incremental lines of von Ebner: Daily, 4mm of organic matrix is deposited. Also every 5 days the arrangement of collagen fibers changes. This creates the incremental lines of von Ebner.
Intratubular dentin

Dentin tubules
S-shaped in the coronal aspect, straight in root dentin

Von Korff fibers
They are an artifact

SURFACES OF THE TEETH

Facial, Mesial, Distal, Lingual, and Incisal Surfaces

• The facial is the surface of a tooth that "faces" toward the lips or cheeks. When there is a requirement to be more specific, terms like labial and buccal are used. The labial is the surface of an anterior tooth that faces toward the lips. The buccal is the surface of a posterior tooth that faces toward the cheek.
• The mesial is the proximal surface closest to the midline of the arch. The distal is the opposite of mesial. The distal is the proximal surface oriented away from the midline of the arch.
• The lingual is the surface of an anterior or posterior tooth that faces toward the tongue. Incisal edges are narrow cutting edges found only in the anterior teeth (incisors). Incisors have one incisal edge
• Proximal Surfaces

A tooth has two proximal surfaces, one that is oriented toward the midline of the dental arch (mesial) and another that is oriented away from the midline of the arch (distal).

Gingiva

The connection between the gingiva and the tooth is called the dentogingival junction. This junction has three epithelial types: gingival, sulcular, and junctional epithelium. These three types form from a mass of epithelial cells known as the epithelial cuff between the tooth and the mouth.

Much about gingival formation is not fully understood, but it is known that hemidesmosomes form between the gingival epithelium and the tooth and are responsible for the primary epithelial attachment. Hemidesmosomes provide anchorage between cells through small filament-like structures provided by the remnants of ameloblasts. Once this occurs, junctional epithelium forms from reduced enamel epithelium, one of the products of the enamel organ, and divides rapidly. This results in the perpetually increasing size of the junctional epithelial layer and the isolation of the remenants of ameloblasts from any source of nutrition. As the ameloblasts degenerate, a gingival sulcus is created.

MAXILLARY FIRST BICUSPID (PREMOLARS)

It is considered to be the typical bicuspid. (The word "bicuspid" means "having two cusps.")

Facial: The buccal surface is quite rounded and this tooth resembles the maxillary canine. The buccal cusp is long; from that cusp tip, the prominent buccal ridge descends to the cervical line of the tooth.

Lingual: The lingual cusp is smaller and the tip of that cusp is shifted toward the mesial. The lingual surface is rounded in all aspects.

Proximal: The mesial aspect of this tooth has a distinctive concavity in the cervical third that extends onto the root. It is called variously the mesial developmental depression, mesial concavity, or the 'canine fossa'--a misleading description since it is on the premolar. The distal aspect of the maxillary first permanent molar also has a developmental depression. The mesial marginal developmental groove is a distinctive feature of this tooth.

Occlusal: There are two well-defined cusps buccal and lingual. The larger cusp is the buccal; its cusp tip is located midway mesiodistally. The lingual cusp tip is shifted mesially. The occlusal outline presents a hexagonal appearance. On the mesial marginal ridge is a distinctive feature, the mesial marginal developmental groove.

Contact Points;The distal contact area is located more buccal than is the mesial contact area.

Root Surface:-The root is quite flat on the mesial and distal surfaces. In about 50 percent of maxillary first bicuspids, the root is divided in the apical third, and when it so divided, the tips of the facial and lingual roots are slender and finely tapered.

Nerve and vascular formation

Frequently, nerves and blood vessels run parallel to each other in the body, and the formation of both usually takes place simultaneously and in a similar fashion. However, this is not the case for nerves and blood vessels around the tooth, because of different rates of development.

Nerve formation

Nerve fibers start to near the tooth during the cap stage of tooth development and grow toward the dental follicle. Once there, the nerves develop around the tooth bud and enter the dental papilla when dentin formation has begun. Nerves never proliferate into the enamel organ

Vascular formation

Blood vessels grow in the dental follicle and enter the dental papilla in the cap stage. Groups of blood vessels form at the entrance of the dental papilla. The number of blood vessels reaches a maximum at the beginning of the crown stage, and the dental papilla eventually forms in the pulp of a tooth. Throughout life, the amount of pulpal tissue in a tooth decreases, which means that the blood supply to the tooth decreases with age. The enamel organ is devoid of blood vessels because of its epithelial origin, and the mineralized tissues of enamel and dentin do not need nutrients from the blood.