Sterilization in Dental Practice

Sterilization is a critical process in dental practice, ensuring that all forms of life, including the most resistant bacterial spores, are eliminated from instruments that come into contact with mucosa or penetrate oral tissues. This guide outlines the accepted methods of sterilization, their requirements, and the importance of biological monitoring to ensure effectiveness.

Sterilization: The process of killing all forms of life, including bacterial spores, to ensure that instruments are free from any viable microorganisms. This is essential for preventing infections and maintaining patient safety.

Accepted Methods of Sterilization

There are four primary methods of sterilization commonly used in dental practices:

A. Steam Pressure Sterilization (Autoclave)

• Description: Utilizes steam under pressure to achieve high temperatures that kill microorganisms.
• Requirements:
  • Temperature: Typically operates at 121-134°C (250-273°F).
  • Time: Sterilization cycles usually last from 15 to 30 minutes, depending on the load.
  • Packaging: Instruments must be properly packaged to allow steam penetration.

B. Chemical Vapor Pressure Sterilization (Chemiclave)

• Description: Involves the use of chemical vapors (such as formaldehyde) under pressure to sterilize instruments.
• Requirements:
  • Temperature: Operates at approximately 132°C (270°F).
  • Time: Sterilization cycles typically last about 20 minutes.
  • Packaging: Instruments should be packaged to allow vapor penetration.

C. Dry Heat Sterilization (Dryclave)

• Description: Uses hot air to sterilize instruments, effectively killing microorganisms through prolonged exposure to high temperatures.
• Requirements:
  • Temperature: Commonly operates at 160-180°C (320-356°F).
  • Time: Sterilization cycles can last from 1 to 2 hours, depending on the temperature.
  • Packaging: Instruments must be packaged to prevent contamination after sterilization.

D. Ethylene Oxide (EtO) Sterilization

• Description: Utilizes ethylene oxide gas to sterilize heat-sensitive instruments and materials.
• Requirements:
  • Temperature: Typically operates at low temperatures (around 37-63°C or 98.6-145°F).
  • Time: Sterilization cycles can take several hours, including aeration time.
  • Packaging: Instruments must be packaged in materials that allow gas penetration.

Considerations for Choosing Sterilization Equipment

When selecting sterilization equipment, dental practices must consider several factors:

• Patient Load: The number of patients treated daily will influence the size and capacity of the sterilizer.
• Turnaround Time: The time required for instrument reuse should align with the sterilization cycle time.
• Instrument Inventory: The variety and quantity of instruments will determine the type and size of sterilizer needed.
• Instrument Quality: The materials and construction of instruments may affect their compatibility with certain sterilization methods.

Biological Monitoring

A. Importance of Biological Monitoring

• Biological Monitoring Strips: These strips contain spores calibrated to be killed when sterilization conditions are met. They serve as a reliable weekly monitor of sterilization effectiveness.

B. Process

• Testing: After sterilization, the strips are sent to a licensed reference laboratory for testing.
• Documentation: Dentists receive independent documentation of monitoring frequency and sterilization effectiveness.
• Failure Response: In the event of a sterilization failure, laboratory personnel provide immediate expert consultation to help resolve the issue.